Uncapped duo Ashton Hewitt and Aaron Wainwright were called up to the Wales squad for their June Tests as a Premiership trio were released.
The Dragons pair and Ospreys prop Rhodri Jones were added to the squad for Tests against South Africa (June 2) and Argentina (June 9 and 16).
The trio were called up as replacements for Worcester Warriors' Josh Adams, Exeter Chiefs' Tomas Francis and Bath's Luke Charteris, who would not be released by their Premiership clubs for the South Africa clash – which falls outside the Test window.
"We are really disappointed that Josh, Tomas and Luke have to miss out on the opportunity to tour with Wales and play international rugby this summer," Wales head coach Warren Gatland said.
"It was a great opportunity for them to play for their country, press their claim in an important year and it is a shame supporters will not get to see them in the red of Wales.
"The situation is very clear for players playing in England going forward and heading into a RWC [Rugby World Cup] year.
"With the players unavailable to link up with the squad until the week of our second Test match we have had to replace them in the squad."
Cardiff Blues' Josh Navidi has also been ruled out after suffering a dislocated shoulder in his team's European Challenge Cup final win over Gloucester on Friday.
Forwards: Rob Evans, Alun Wyn Jones, Nicky Smith, Elliot Dee, Ryan Elias, Rhodri Jones, Samson Lee, Dillon Lewis, Adam Beard, Bradley Davies, Seb Davies, Cory Hill (co-captain) James Davies, Ellis Jenkins (co-captain), Ross Moriarty, Aaron Wainwright, Aaron Shingler.
Backs: Aled Davies, Gareth Davies, Tomos Williams, Gareth Anscombe, Rhys Patchell, Hadleigh Parkes, Owen Watkin, Scott Williams, Hallam Amos, Steff Evans, Ashton Hewitt, George North, Tom Prydie.
26. September 2018
|Great Britain and Argentina handed Davis Cup wildcards|
|Strong start key for European skipper Bjorn|
|Schmiedlova ousts former Tashkent champion Hibino|
|Mourinho and Pogba in frosty training ground exchange|
|Ashworth to leave FA for Brighton job|
|Gillespie backs Finch for Test success|
|Woods and Mickelson pairing ´not too likely´ - Furyk|
|Pogba v Mourinho: Who should stay and who should go at Manchester United?|
|Rahm better than I was at my first Ryder Cup, says McIlroy|
|Erdogan claims double standards in Ozil controversy|
|F1 Raceweek: Mercedes eye five in a row in Sochi – Russian GP in numbers|
|Rose: Stature doesn´t win Ryder Cup points|
|Sonny Bill Williams fit to face Pumas - Crotty|
|Exuberant Rahm expecting to be paired with calming influence|
|I´ve proved myself over and over - Garcia|
|Ocon negotiating with Williams for 2019|
|Kvitova´s Wuhan quest ended by Pavlyuchenkova|
|Hummels impressed by ´incredible´ Sanches|
|Brewers benefit from Pirates´ trade deadline acquisition of Archer|
|Barty upsets Kerber in Wuhan|
|England could still pick Cipriani despite training camp snub|
|Astros become first defending champions to reach 100 wins since 1990 Athletics|
|Wilder waiting for ´real offer´ to fight Joshua|
|LeBron focusing on patience after first Lakers practice|
|Pochettino expects Spurs in new stadium by Christmas|
|Inzaghi: With Ronaldo, Juve are best team in Europe|
|Some people talk too much – Icardi hits back at critics|
|Kovac, Robben praise Augsburg after draw|
|Angels star Ohtani to undergo Tommy John surgery|
|Simeone tips Gimenez to be fit for Madrid derby|
|Smith on his NBA Finals miscue: People make mistakes all the time|
|Rams CB Talib to have ankle surgery|
|Cousins ´feeling a lot better´, still unsure when he´ll return|
|I knew we´d be in trouble with Jones on penalties – Mourinho|
|Coyotes forward Galchenyuk expected to miss opener|
|Guardiola won´t compare Foden to Iniesta|
|Lampard shell-shocked as Derby win at Old Trafford|
|Ryder Cup 2018: USA celebrities take the honours|
|Manchester United´s torrid penalty shoot-out run continues against Derby|
|Guardiola: Foden already a great, great player|
25. September 2018
|Atletico Madrid 3 Huesca 0: Rojiblancos cruise ahead of Madrid derby|
|EFL Cup: Lampard dumps out Mourinho, Burnley lose at Burton Albion|
|Mourinho denies Pogba row despite stripping midfielder of vice-captaincy|
|Fabinho situation not complicated, insists Klopp|
|Manchester United 2 Derby County 2 (Derby win 8-7 on pens): Lampard heaps pressure on Mourinho|
|Afghanistan snatch tie after Shahzad pummels India|
|Inter 2 Fiorentina 1: D´Ambrosio maintains Nerazzurri revival|
|Oxford United 0 Manchester City 3: Foden stars as EFL Cup holders cruise into round four|
|Cousin takes Gabon post after Aubameyang rejection|
|Bayern Munich 1 Augsburg 1: Neuer error costs champions|
|Paris Saint-Germain must be a Champions League force to help Neymar - Baptista|
|Salgado warns of ´war´ in Spanish football|
|Cech deserves Arsenal place, says Seaman|
|Halep and Garcia among seeds to fall in Wuhan|
|F1 Raceweek: Mercedes taking no comfort in points advantage|
|Benitez charged by FA for referee comment relating to Zaha treatment|
|Cibulkova dumps out hampered Halep in Wuhan|
|Mourinho questions his players amid reports Pogba won´t captain United again|
|European Ryder Cup diary: Fleetwood´s hair delights Poulter|
|USA Ryder Cup diary: Tiger´s roaring reception, McEnroe´s bold prediction|
|Loans to be limited under new FIFA proposal|
|Prolific Nadolo signs new Montpellier contract|
|Modric? For us, Messi is the best – Valverde|
|Murray makes winning start in Shenzhen|
|Mickelson would cherish first victory on European soil|
|Hodge hoping pressure proves too much for South Africa|
|Woodward in show of support for under-fire Mourinho|
|Mickelson jokes he wants ´a shot a side´ from Tiger in Thanksgiving showdown|
|Reed: Captain America is a villain in Europe|
|Duminy to captain Proteas as Elgar replaces injured Amla|