The Association of Spanish Footballers (AFE) has confirmed its formal opposition to LaLiga's plan to play Girona's home game against Barcelona in Miami.
Both clubs have agreed to the proposal, which comes as part of a deal with Relevant, the company behind pre-season tournament the International Champions Cup.
LaLiga matches will be played in the United States across a 15-year period under the terms of the agreement, with Girona-Barcelona slated for January 2019.
But players have signalled their opposition to the scheme, while Real Madrid president Florentino Perez indicated Los Blancos would refuse to take part in a match outside of Spain.
The Royal Spanish Football Federation (RFEF) has asked for more documentation from LaLiga over the proposal, but the league's president Javier Tebas still believes the match will take place in Miami.
The AFE previously threatened strike action over the Relevant deal and on Monday the organisation confirmed it will not give the green light to the Miami plan.
A statement read: "The AFE want to express the following after receiving a more detailed report on the game between Girona and Barcelona which LaLiga hopes to move to the United States in 2019:
"1. We have informed LaLiga that said report does not specify the specific and necessary objective elements to be able to determine that the provisions of Articles 8, 9 and 37 of the Collective Bargaining Agreement will not be violated.
"2. The AFE reminds [LaLiga] that for the time being, there are no mandatory authorisations that allow for the game to be played in the United States.
"3. For that reason, considering the lack of information mentioned, the AFE has transmitted to LaLiga its lack of support for that decision."
AFE additionally confirmed the body will not consider changes to its agreement with LaLiga until 2020.
RFEF leader Luis Rubiales opposes the Relevant deal, describing as an "invasion" of the United States, but Tebas has cited the precedent of this season's Supercopa de Espana between Barca and Sevilla being played in Morocco.
23. Oktober 2018
|Green: Warriors have to clean up defensively|
|Mariners say Bolt talks ongoing amid reports contract offer was rejected|
|Proud captain Ozil revels in Arsenal´s ´sexy football´|
22. Oktober 2018
|Puel angry over Holding handball|
|Cilic breezes through in Basel, Anderson fights back|
|Ingram: Four-game ban better than we expected|
|Raiders send Cooper to Cowboys for first-round pick|
|Arsenal 3 Leicester City 1: Outstanding Ozil inspires comeback win|
|World Series 2018: Three reasons why the Dodgers will win|
|Yarmolenko out for six months after Achilles surgery|
|World Series 2018: Three reasons why the Red Sox will win|
|Ozil eclipses Klinsmann to become Premier League´s most prolific German|
|Allegri expecting Old Trafford battle against Mourinho´s United|
|Bertens feared she did not belong in Kerber clash|
|Returning Ronaldo expecting ´tough´ game against Manchester United|
|Cristiano Ronaldo confident of clearing his name amid rape allegation|
|Osaka looking to learn from losing start in Singapore|
|Kovac expects tough test against AEK Athens|
|Whyte gives Chisora deadline over rematch|
|Spartak Moscow sack Massimo Carrera|
|Atletico Madrid confirm fresh injury issue for Gimenez|
|Dolberg extends Ajax contract after Real Madrid links|
|Ronaldo an example for Lukaku to follow|
|Bertens battles back to blast past Kerber|
|Ramos apologises for training ground fracas|
|Raikkonen should take heed of Alonso misfortune as F1 career winds down|
|Guardiola wants Manchester City fans to push team to Champions League glory|
|Isco not interested in departed Ronaldo - he didn´t want to be here|
|World Series 2018: Three storylines to watch in Red Sox-Dodgers|
|Neymar focus bad for football - PSG´s Maxwell|
|Marco Ianni charged by FA over Jose Mourinho touchline row|
|Stephens sees off Osaka in see-saw Singapore battle|
|I´m happy at Manchester United - Mourinho dismisses Real Madrid reports|
|Semenya and Asher-Smith head IAAF Female World Athlete of the Year shortlist|
|Mandzukic to miss Manchester United trip|
|I turned down Manchester United to return to Juventus - Bonucci|
|Sanchez out of Manchester United´s clash with Juventus|
|Australia make Short work of run chase in T20 win|
|Brotherly g-love! Tommy Fury follows Tyson into the pros|
|Ozil´s international retirement can help Arsenal - Emery|
|If you sack Lopetegui, you have to sack us all – Isco|
|Lopetegui defiant amid Real Madrid sack reports|
|Sri Lanka spinner Herath to retire after Galle Test|
|Ronaldo returns for Old Trafford welcome amid disquieting noise|
|Garcia claims third Andalucia Valderrama Masters title|
|Ex-England international Waldrom retires|
|´Massive personality´ Hart can lift Burnley, says Kompany|
|Liverpool felt wrath of ´emotional´ Klopp, says Shaqiri|
|Reid: Jenkins capitalised on Kaepernick movement|
|Manchester United v Juventus: Ronaldo out to end dismal scoring record against Mourinho|
|Injured Ribery to miss Bayern´s clash with AEK|
|Wilshere would have stayed at Arsenal under Wenger|
|Mahomes stars again as Chiefs crush Bengals|
|Bolt must accept ´much less´ to join Mariners, says A-League chief|
|Klopp compares Liverpool to dog without favourite toy|
|Young leads Hawks to first win, Thunder fall in Westbrook´s return|
|Spalletti hits out at physical Milan – I had three players asking to be replaced|
|Cowboys, Ravens lose after missed kicks in final seconds|
|Hernangomez secures Nuggets´ win over Warriors with last-second block|
|Hasler returns to Manly on three-year deal|
|Sarri: Chelsea fans must respect Mourinho|
|Ronaldo learnt from Scholes – Pjanic praises Juventus star|
|CA dismisses spot-fixing claims|
|WATCH: Will Ferrell leads chants with LAFC fans|
|MLS Review: Galaxy on track for play-offs as Timbers, DC clinch spots|
|Elliott secures win at Hollywood Casino 400|
|Thunder´s Westbrook makes season debut against Kings|
|Saints beat Ravens after Tucker misses extra point|
|Rondo, Paul and Ingram banned after Lakers-Rockets fight|
|Saints QB Brees reaches another historic milestone|